Der neue Spinmarker für Proteine, PaNDA. Er kann mittels einer Diels-Alder-Reaktion mit inversem Elektronenbedarf (DAinv) an genetisch codierte nichtkanonische Aminosäuren in einem Protein gebunden werden. Bild: Anandi Kugele

New protein spin labelling technique

University of Konstanz researchers develop a new site-directed spin labelling approach based on genetically encoded noncanonical amino acids amenable to Diels-Alder chemistry as well as a new spin label, PaNDA

Site-directed spin labelling (SDSL) used in combination with electron paramagnetic resonance (EPR)
spectroscopy has been a tried and trusted technique for elucidating the structure, function and
dynamics of proteins and protein complexes. Nitroxide-based spin labels are among the most popular and best established ones because they are small, non-disturbing and exhibit excellent spectroscopic properties. “Ideal spin labelling procedures exhibit high reaction rates and selectivity”, explains Professor Malte Drescher, Professor for Spectroscopy of Complex Systems at the University of Konstanz’s Department of Chemistry and main author on the study alongside Professor Valentin Wittmann, who specializes in organic synthesis. Read more...